Detailed Math:
';steps+='1. Total Peptide: '+mg+'mg × 1000 = '+totalMcg.toLocaleString()+' mcg
';steps+='2. Concentration: '+totalMcg.toLocaleString()+' mcg / '+ml+'mL = '+mcgPerMl.toLocaleString()+' mcg/mL
';steps+='3. Per Unit (100-unit scale): '+mcgPerMl.toLocaleString()+' / 100 = '+mcgPerUnit.toFixed(1)+' mcg per unit
';steps+='4. Units for '+mcg+'mcg Dose: '+mcg+' / '+mcgPerUnit.toFixed(1)+' = '+tickDose.toFixed(1)+' Units';document.getElementById('stepDetail').innerHTML=steps;document.getElementById('stepDetail').style.display='block';}else{document.getElementById('stepDetail').style.display='none';}}
How to Use the Peptide Calculator
The peptide calculator is an essential tool for researchers and individuals needing to accurately reconstitute lyophilized (freeze-dried) peptides. Because peptides are often measured in milligrams (mg) but administered in micrograms (mcg), calculating the exact volume of liquid to draw into a syringe can be complex and prone to human error.
This tool simplifies the math by converting your dry peptide mass and the volume of bacteriostatic water added into a precise "units" measurement for standard insulin syringes.
- Peptide Amount (mg)
- The total weight of the dry peptide powder inside the vial (e.g., 2mg, 5mg, 10mg).
- Bacteriostatic Water (mL)
- The amount of diluent you inject into the vial to dissolve the powder.
- Desired Dose (mcg)
- The specific amount of active peptide you intend to use for a single application.
How It Works: The Reconstitution Formula
To understand how the peptide calculator reaches its result, you must follow the conversion from mass to concentration. The primary formula used is:
Dose Units = Desired Dose (mcg) / [(Total mg × 1000) / (ml Water × 100)]
- Step 1: Convert mg to mcg (1mg = 1000mcg).
- Step 2: Determine concentration per mL (Total mcg / mL of water).
- Step 3: Calculate mcg per "Unit" on the syringe. Most syringes have 100 units per 1mL.
- Step 4: Divide the target dose by the mcg-per-unit value to find your pull mark.
Calculation Example
Example Scenario: You have a 5mg vial of BPC-157 and you add 2mL of bacteriostatic water. You want to administer a dose of 250mcg using a standard 100-unit syringe.
Step-by-step solution:
- Convert Mass: 5mg × 1000 = 5000mcg.
- Calculate Concentration: 5000mcg / 2mL = 2500mcg per mL.
- Calculate Per Unit: 2500mcg / 100 units = 25mcg per unit.
- Final Dose: 250mcg / 25mcg per unit = 10 Units.
- Result: Draw the liquid to the 10 unit mark on your syringe.
Common Questions
What is the best water to use for reconstitution?
Bacteriostatic water (sterile water with 0.9% benzyl alcohol) is typically preferred. The alcohol acts as a preservative, preventing the growth of bacteria, which allows the reconstituted peptide vial to be stored in the refrigerator for several weeks. Plain sterile water does not have this preservative and should be used for single-use only.
Why does the syringe size matter?
Most insulin syringes are "U-100" syringes, meaning there are 100 units in 1mL of volume. However, some syringes are 0.5mL (50 units) or 0.3mL (30 units). While the amount of peptide per "unit" stays the same if the concentration is the same, the capacity of the syringe determines how much total liquid you can draw at once.
Does more water make the peptide stronger?
No. Adding more water dilutes the peptide, meaning you have to draw more liquid into the syringe to get the same dose in micrograms. Adding less water creates a more concentrated solution, meaning you draw less liquid. The total amount of peptide (the mg in the vial) remains constant regardless of how much water you add.
How should I store my peptides after using the peptide calculator?
Once reconstituted, most peptides become fragile. They should be stored in a cool, dark place (usually a refrigerator between 2°C and 8°C). Avoid freezing reconstituted peptides, as the expansion of ice crystals can damage the delicate molecular structure of the peptide chains.